Tumor Cell Behavior in Porous Hydrogels: Effect of Application Technique and Doxorubicin Treatment

Abstract

© 2019, Springer Science+Business Media, LLC, part of Springer Nature. The effect of porosity on diffusion characteristics of scaffolds and invasive capacity of MCF-7 and PC-3 tumor cells was studied for gelatin hydrogels. According to MTS test results, the efficiency of population of a macroporous cryogel by cells applied by different techniques increased in the following order: migration from the monolayer<surface adhesion<<injection. Tumor cells in the cryogel differed by the migration and aggregation activity; injection route ensured a more uniform and dense population. In the cryogel-based culture, the cytotoxic effect of doxorubicin was 3-lower than in monolayer culture, which can be explained by supporting effect of the scaffold on cell growth and clustering. The results are of interest for the creation of tumor models and grafts with controlled properties.