Molecular methods in plant disease diagnostics: principles and protocols/ edited by Neil Boonham, Jenny Tomlinson and Rick Mumford.

Abstract

This book provides protocols for nucleic acid-based methods currently applied to plant pathogen detection and identification, showing that great strides have been made in molecular diagnostics since those first tentative steps back in the late 1980s/early 1990s. It takes the practitioner through the full range of molecular diagnostic and detection methods and, as these generic techniques are appropriate for use on any target with minimal modification, also provides a useful resource for students of plant pathology and plant pathologists. Beginning with the background and future directions of the science, it then addresses DNA barcoding, microarrays, polymerase chain reactions (PCR), quality assurance and more, forming a complete reference on the subject.