Analysis of changes in the gastrointestinal mictoflora in normal and abnormal state and identification of individual strains of possible inflammatory disease agents

Abstract

© 2019 Christa et al. The total content of anaerobic bacteria in the stool samples was determined in normal conditions and in inflammatory diseases (ulcerative colitis and Crohn’s disease). An increase in the total number of microorganisms in the inflammatory process has been established. The number of cells in the stool samples of patients with ulcerative colitis increased 1.2-1.4 times, and in Crohn’s disease-1.7-1.9 times compared with the norm and amounted to 7.1 ± 2.1•1010 CFU/g and 11.0 ± 2.8 •1010 CFU/g respectively. Using mass spectrometric analysis with a Bruker Daltonik MALDI Biotyper mass spectrometer, a significant increase in the number of E. coli bacteria and other Escherichia bacteria was shown. An actively growing bacterial strain, identified by analysis of the 16 S rRNA nucleotide sequences as species Escherichia fergusonii G2, was isolated from stool samples from patients with Crohn’s disease.