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Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice

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dc.contributor Казанский федеральный университет
dc.contributor.author Shafigullina Aygul Kasyjmovna
dc.contributor.author Kaligin Maxim Sergeevich
dc.contributor.author Mavlikeev Mikhail Olegovich
dc.contributor.author Chernova Olga Nikolaevna
dc.contributor.author Yakovleva Olga Vladislavovna
dc.contributor.author Titova Angelina Andreevna
dc.contributor.author Rizvanov Albert Anatolevich
dc.contributor.author Kiyasov Andrey Pavlovich
dc.date.accessioned 2019-11-07T11:41:13Z
dc.date.available 2019-11-07T11:41:13Z
dc.date.issued 2019
dc.identifier.citation Shafigullina A.K. Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice / A.K. Shafigullina, M.S. Kaligin, M.O. Mavlikeev, O.N. Chernova, O.V. Yakovleva, A.A. Titova, A.A. Rizvanov, A.P. Kiyasov // Annual Congress of European Society of Gene and Cell Therapy, 22-25 October 2019, Barcelona, Spain. - HUMAN GENE THERAPY: (P470). - P.156-157.
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/151976
dc.description.abstract One of diabetes mellitus (DM) animal models is performed by injection of streptozocin-nicotinamide (SZC-NA) - agents that via GLUT-2 transporter blockage affect glucose transport into cells. GLUT-2 is highly present on pancreatic β-cells that explain toxicity of STZ to them and application of SZN-NA to model DM. GLUT-2 is present also on hepatocytes, which participate in maintaining glucose levels and deposit it as glycogen. Damage of hepatocytes is always accompanied by activation of hepatic stellate cells (HSC) that transform into collagen-producing myofibroblasts, resulting in liver fibrosis. The aim of the research was to study reaction of liver cells to SZN-NA-induced DM in mice. Experimental groups: 1) control mice (C57Bl/J); 2) mice with intraperitoneal injection of SZC-NA (100mg/kg). Liver paraffin sections (40 days after) were stained by Mallory's trichrome (connective tissue), PAS (glycogen content), immunohistochemically (IHC) with antibodies to CD163 (macrophages), desmin (HSC), α-SMA (myofibroblasts). Diabetes development was proved by increase of blood sugar and decrease of insulin-secreting β-cells in pancreas. In the liver there was hydropic degeneration of hepatocytes with reduction of glycogen content, mixed polymorphonuclear-mononuclear infiltration including CD163+macrophages. IHC to desmin demonstrated reduced number of desmin+HSC in compare to control group. IHC to α-SMA showed no myofibroblasts. Features of fibrosis were excluded by Mallory's trichrome staining to connective tissue. Thus, SCZ-NA-induced DM is accompanied by damage of glycogen content in hepatocytes, inflammation with Kupffer macrophages activation. The HSC are inhibited, they don`t transform into myofibroblasts, there is no liver fibrosis development. Work supported by Program of Competitive Growth of KFU.
dc.language.iso en
dc.relation.ispartofseries HUMAN GENE THERAPY
dc.rights открытый доступ
dc.subject streptozocin-nicotinamide
dc.subject diabetes mellitus
dc.subject liver
dc.subject hepatic stellate cells
dc.subject myofibroblasts
dc.subject macrophages
dc.subject.other Медицина и здравоохранение
dc.title Reaction of liver cells to streptozocin-nicotinamide-induced diabetes mellitus in mice
dc.type Article
dc.contributor.org Институт фундаментальной медицины и биологии
dc.description.pages 156-157
dc.pub-id 213549
dc.identifier.doi 10.1089/hum.2019.29095.abstracts


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