Indication and Identification of Bacillus anthracis Isolates from the Middle Volga Region by Multi-Primer PCR

Abstract

© 2017, Springer Science+Business Media, LLC, part of Springer Nature. The article describes the results of improved preparation of various object sampling for indication, identification and genotyping of Bacillus anthracis isolates with molecular genetic methods based on multilocus sequence typing using specific primers. Multi-primer PCR used enabled the detection and differentiation of B. anthracis isolates. The used primers allowed to differentiate isolates by their belonging to the genus Bacillus and B. anthracis species by the 1 plasmid presence. The detection of the рХ02 plasmid encoding for capsule formation indicates pathogenicity of the isolates studied. Using multi-primer PCR allowed to determine the epizootic situation of anthrax in the Middle Volga Region and to establish that two genotypic groups of the pathogen B. anthracis circulate in the region.