Parenchymal and nonparenchymal cellular proliferation dynamics after partial hepatectomy with and without 2-Acetylaminofluorene injection in rats

Abstract

Restoration of liver after partial hepatectomy (PH) depends on proliferation dynamics of parenchymal and nonparenchymal liver cell populations. To study separately proliferation impact of nonparenchymal cells it is recommended to inhibit hepatocytes proliferation by injection of acetoaminofluorene (AAF). The aim of the research was to study dynamics of parenchymal and nonparenchymal cells proliferation after PH w/wo AAF injection. Experimental groups were 1) PH; 2) PH with intraperitoneal AAF injection (0,2ml of 1.5g/100ml). Liver paraffin slices (1,5,7,14,21,28 days after transplantation) were stained with antibodies against proliferation marker Ki-67. AAF injection inhibits proliferation of parenchymal and nonparenchymal cells, has severe effect on central vein region, leads to reduction of liver regeneration (cellular dystrophy and degeneration on 28th days). Work supported by Program of Competitive Growth of KFU.