Characterization of growth-promoting activity of Pseudomonas Putida Strain MG-2.

Abstract

The present study was conducted to isolate and characterize the native plant growth-promoting bacterium from potato rhizosphere in the Republic of Tatarstan, Russia. Based on the gyrB (DNA gyrase subunit B) gene sequence analysis, bacterial isolate was identified as Pseudomonas putida MG-2. Production of indole-3-acetic acid (IAA) was dependent on tryptophan: maximum of IAA accumulation was 11.5 μg/ml on 72nd hour of growth. Furthermore, the ability to synthesize the catechol siderophores, phytate-mobilizing activity and halotolerance were revealed. The treatment of pea and rye seeds by P. putida MG-2 suspension stimulate the seedlings growth and roots biomass by up to 24-28% and 5-14%, respectively. Therefore, this bacterial isolate may be potentially beneficial as growth-promoting factor.