Unfreezing water quantity as a possible way of winter wheat frost resistance appreciation

Abstract

The investigation of unfreezing water quantity change dynamics took place after the plant tissue suffered from the influence of membrane active substances which change water permeability: pipolphen (10-3 M), verapamil (10-4 M), CaCl2 (10-3 M), Gd(NO3)3 (2.5- 10-2 M). The measurements of unfreezing water quantity were made by a simple method on the automatic cogerent «home-made» NMR-plant working at 15 MHz. The objects of investigation were roots of different frost resistant winter wheat (Triticum aestivum) sorts: less frost resistant Mironovskya-808, and more frost resistant Kazanskya-84. The temperature range of unfreezing water quantity was -7° - -30 °C. We got to know that pipolphen and verapamil increase membrane water permeability together with the increase in the quantity of unfreezing water, and the most frost resistant sort Kazanskya-84 had the greatest quantity of unfreezing water in this temperature range. The addition of Ca2+-ions brought about the lowering of unfreezing water. The regularity of influence of the membrane active substances at-30 °C proves that changes in water permeability correlate with those in membrane hydration.