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Factors influencing the cellular location of proteolytic enzymes of Bacillus intermedius

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dc.contributor.author Sharipova M.
dc.contributor.author Shakirov E.
dc.contributor.author Gabdrakhmanova L.
dc.contributor.author Balaban N.
dc.contributor.author Kalacheva N.
dc.contributor.author Rudenskaya G.
dc.contributor.author Leshchinskaya I.
dc.date.accessioned 2018-09-17T21:30:55Z
dc.date.available 2018-09-17T21:30:55Z
dc.date.issued 2000
dc.identifier.issn 1234-1010
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/135090
dc.description.abstract Thiol-dependent serine proteinase and glutamylendopeptidase of Bacillus intermedius 3-19 being prevailing enzymes in the total pool of extracellular proteinases (95%) of this microorganism in catalytic active form were detected on the membrane of the cells. Production of these enzymes was maximum on the medium containing inorganic phosphate and gelatin and decreased 2-4-fold on the medium with glucose and lactate. The level of the activity of extracellular enzymes correlated with that of corresponding membrane-bound proteins. The addition of CoCl2 (2 mM) into the medium caused essential increase in extracellular glutamylendopeptidase activity and promoted the release of membrane-bound enzyme into cultural fluid. Proteolytic activity was detected in cytoplasm also. Proteinases localized in cytoplasm were shown to differ in properties from those secreted.
dc.relation.ispartofseries Medical Science Monitor
dc.subject Glutamylendopeptidase
dc.subject Location
dc.subject Membrane-bound enzymes
dc.subject Thiol-dependent serine proteinase
dc.title Factors influencing the cellular location of proteolytic enzymes of Bacillus intermedius
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 6
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 8
dc.source.id SCOPUS12341010-2000-6-1-SID0034100708


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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