Fluorescent polarization immunoassay for sulphadiazine using a high specificity antibody

Abstract

A rapid and sensitive fluorescent-labelled polarization immunoassay for sulphadiazine (SDZ) is reported. Fluorescein-labelled tracers were synthesized and a specific SDZ antibody has been used in the development of the method. The influence of the fluorescent label type on the immunoassay sensitivity was investigated. Homologous and non-homologous tracer - antiserum combinations were varied to provide increased sensitivity of the immunoassay. A detection limit of 0.2 ng mL-1 for SDZ in 50 μL aqueous sample has been achieved. The cross-reactivity of other sulphonamide drugs has also been examined.