Abstract:
An amperometric enzyme immunosensor for the determination of antigen (Ag) of pathogenic fungi Trichophyton rubrum (TR) was developed. The immunosensor is based on a specific immunoreaction of Ag with antibodies which were immobilized together with cholinesterase into a nitrocellulose membrane (biosensitive part of immunosensor). The biosensitive part could be regenerated and is stable for at least 35 days. The developed immunosensor allows the determination of down to 1x10-15mgml-1 of Ag TR. The total time of immunoassay is less than 20min. The method was applied for the determination of Ag TR in human blood serum. Copyright (C) 2000 Elsevier Science B.V.