Enzyme amperometric sensor for the determination of cholinesterase inhibitors or activators

Abstract

An enzyme amperometric sensor (EAS) based on immobilized cholinesterase (ChE) sensitive to ChE effectors (both specific and non-specific) is shown to be useful in enzyme immunoassay. For example, a mink autoimmune (Aleutian) disease can be diagnosed with the EAS when an antigen is labelled with a ChE inhibitor. When the ChE-containing membrane is modified by incorporation of the antigen to give an immunoenzyme EAS, this immunoassay can be performed on the basis of the steric shielding of the enzyme active sites with the immunocomplexes formed. © 1993.