dc.contributor |
Казанский федеральный университет |
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dc.contributor.author |
Shafigullina Aygul Kasyjmovna |
|
dc.contributor.author |
Titova Angelina Andreevna |
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dc.contributor.author |
Rizvanov Albert Anatolevich |
|
dc.contributor.author |
Gumerova Anisa Azatovna |
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dc.contributor.author |
Kiyasov Andrey Pavlovich |
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dc.date.accessioned |
2018-06-07T06:32:37Z |
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dc.date.available |
2018-06-07T06:32:37Z |
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dc.date.issued |
2018 |
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dc.identifier.citation |
Shafigullina A. Histochemical analysis of self-organizing 3D spheroid-like cultures of adipose-derived mesenchymal stem cells / A. Shafigullina, A. Titova, A. Rizvanov, A. Gumerova, A. Kiassov // 52nd Annual Scientific Meeting of ESCI, 29 May -1 June 2018, Barcelona, Spain. - European Journal of Clinical Investigation. - May, 2018. - Vol. 48 (Suppl.1). - P. 91. |
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dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/131560 |
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dc.description.abstract |
Histochemical staining demonstrated fusion of cells with formation of syncytium-like structures. On the periphery cells were polygonal, round, had epithelial morphology. Groups of cells organized in line inside spheroid remained elongated AD-MSC shape. Most of the cells did not proliferate. There were no migrating SMA+ cells. Polygonal cells on the periphery and a few cells inside the spheroid were desmin+. Interestingly, peripheral and elongated shape cells expressed epithelial marker CK-19+. Probably, within spheroid there was spontaneous mesenchymal-epithelial transdifferentiation. When spheroids were placed in a new culture dish we observed explantational outgrowth of spindle-shaped AD-MSC suggesting that cells within spheroids were alive, able to outgrow and revert through epithelial-mesenchymal transdifferentiation back to MSC phenotype. Investigation was funded by RFBR grant 18-04-01133 and supported by Program of Competitive Growth of KFU. |
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dc.language.iso |
en |
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dc.relation.ispartofseries |
52nd Annual Scientific Meeting of ESCI |
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dc.rights |
открытый доступ |
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dc.subject |
Histochemical analysis |
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dc.subject |
3D spheroid-like cultures |
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dc.subject |
adipose-derived mesenchymal stem cells |
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dc.subject |
mesenchymal-epithelial transformation |
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dc.subject |
epithelial-mesenchymal transformation |
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dc.subject.other |
Медицина и здравоохранение |
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dc.title |
Histochemical analysis of self-organizing 3D spheroid-like cultures of adipose-derived mesenchymal stem cells |
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dc.type |
Thesis |
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dc.contributor.org |
Институт фундаментальной медицины и биологии |
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dc.description.pages |
|
|
dc.pub-id |
181997 |
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