Kazan Federal University Digital Repository

The detection of stem-like circulating tumor cells could increase the clinical applicability of liquid biopsy in ovarian cancer

Show simple item record

dc.contributor.author Gening S.O.
dc.contributor.author Abakumova T.V.
dc.contributor.author Gafurbaeva D.U.
dc.contributor.author Rizvanov A.A.
dc.contributor.author Antoneeva I.I.
dc.contributor.author Miftakhova R.R.
dc.contributor.author Peskov A.B.
dc.contributor.author Gening T.P.
dc.date.accessioned 2022-02-09T20:44:01Z
dc.date.available 2022-02-09T20:44:01Z
dc.date.issued 2021
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/169897
dc.description.abstract Stem properties allow circulating tumor cells (CTCs) to survive in the bloodstream and initiate cancer progression. We aimed to assess the numbers of stem-like CTCs in patients with ovarian cancer (OC) before treatment and during first-line chemotherapy (CT). Flow cytometry was performed (Cytoflex S (Beckman Coulter, CA, USA)) using antibodies against CD45; epithelial markers EpCAM and cytokeratin (CK) 8,18; mesenchymal vimentin (vim); and stem-like CD44, CD133 and ALDH. This study included 38 stage I–IV OC patients (median age 66 (Q1–Q3 53–70)). The CK+vim-counts were higher (p = 0.012) and the CD133+ALDHhigh counts were lower (p = 0.010) before treatment in the neoadjuvant CT group than in the adjuvant group. The patients with ascites had more CK+vim-cells before treatment (p = 0.009) and less EpCAM-vim+ cells during treatment (p = 0.018) than the patients without ascites. All the CTC counts did not differ significantly in paired samples. Correlations were found between the CK-vim+ and CD133+ALDHhigh (r = 0.505, p = 0.027) and EpCAM-vim+ and ALDHhigh (r = 0.597, p = 0.004) cells before but not during treatment. Multivariate Cox regression analysis showed that progression-free survival was longer with the presence of surgical treatment (HR 0.06 95% CI 0.01–0.48, p = 0.009) and fewer CD133+ALDHveryhigh cells (HR 1.06 95% CI 1.02–1.12, p = 0.010). Thus, CD133+ALDH+ CTCs have the greatest prognostic potential in OC among the phenotypes studied.
dc.subject ALDH
dc.subject CD133
dc.subject CD44
dc.subject Circulating tumor cells
dc.subject CTCs
dc.subject Cytokeratin
dc.subject EpCAM
dc.subject Ovarian cancer
dc.subject Stem cells
dc.subject Vimentin
dc.title The detection of stem-like circulating tumor cells could increase the clinical applicability of liquid biopsy in ovarian cancer
dc.type Article
dc.relation.ispartofseries-issue 8
dc.relation.ispartofseries-volume 11
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2021-11-8-SID85113170662


Files in this item

This item appears in the following Collection(s)

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics