The construction of supramolecular and hybrid Ag-AgCl nanoparticles with photodynamic therapy action on the base of tetraundecylсalix[4]resorcinarene-mPEG conjugate

Abstract

The development of new nanomaterials with therapeutic potential is a modern task due to their outstanding properties and promising effectiveness. Here the usage of acylhydrazone bond-based сalix[4]resorcinarene-mPEG conjugate (C11-mPEG) in the formation of both supramolecular and hybrid nanoparticles with in vitro photodynamic therapy (PDT) activity is described. C11-mPEG is hydrolyzed with a decrease in pH of solution; the conjugate and its degradation product have the low hemolytic activity and low cytotoxicity against normal cells. The self-association of the conjugate was studied in the aqueous solutions with pH 7.4 and 5. C11-mPEG forms self-associates that serve as pH-sensitive supramolecular nanocontainers for photosensitizer Methylene Blue (MB). In the in vitro PDT experiment the stronger decrease of tumor cells (M-Hela cells) viability in the presence of encapsulated MB is observed in comparison with free MB. Also C11-mPEG acts as a stabilizing agent for Ag@AgCl nanoparticles, which were obtained with 5/1 and 2/1 Ag+/C11-mPEG molar ratio and different reducing agents (sodium borohydride and hydrazine hydrate). The stronger cytotoxicity of hybrid NPs against tumor cells in comparison with normal was shown. Further it was found that the irradiation of the hybrid nanoparticles at 630 nm leads to the sharp increase in their cytotoxicity against M-Hela cells. The high level of ROS formation in the cells in the presence of both supramolecular and hybrid NPs under in vitro PDT experiment was determined.