Электронный архив

Analysis of the intestinal microbiome in colorectal cancer

Показать сокращенную информацию

dc.contributor.author Gataullin B.I.
dc.contributor.author Gataullin I.G.
dc.contributor.author Nga N.T.
dc.contributor.author Kolpakov A.I.
dc.contributor.author Ilinskaya O.N.
dc.date.accessioned 2022-02-09T20:33:54Z
dc.date.available 2022-02-09T20:33:54Z
dc.date.issued 2021
dc.identifier.issn 0368-4814
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/169049
dc.description.abstract Aim. To conduct a comparative analysis of the microbiome of biopsies of a tumor and normal intestinal epithelium of patients diagnosed with colorectal cancer and to identify of functional activities of the obtained bacterial isolates that affect the development of the tumor. Methods. The study included 50 patients with malignant neoplasms of the colon: 36 men and 24 women. The mean age of the patients was 64.1±10.2 years. To analyze the microbiota of the biopsies, DNA samples were obtained from the tissue of the unaffected colon mucosa and tumor of the patients. Bacterial 16S rRNA genes fragments were amplified using bar-coded primer bakt_341f. Metagenomic next-generation sequencing was performed using the MiSeq platform (Illumina, USA). The obtained data were processed by bioinformatic methods using the QIIME package. Recognition of microorganisms depending on the morphotype and gram staining of the microflora was carried out using combination differential media and biochemical tests. Statistical analysis was carried out using Microsoft Excel, Service Pack 2 for Office XP, Statistica 6.0 (StatSoft). A comparative analysis was performed with the Student's t-test and the Mann-Whitney test in case of unmet conditions of validity. Alpha diversity of bacterial communities was quantified by the Shannon diversity index and the UniFrac distance for beta diversity analysis. Results. In patients with colorectal cancer, 5 bacterial phyla were isolated, the predominant of which were Firmicutes and Bacteroidetes, while the content of Actinobacteria was low. In addition, a higher number of representatives of Fusobacteria was observed in the tumor tissue compared to the tissue of a healthy mucosa, at a distance of 5 centimeters proximal to the tumor. The results of this study indicate that the microbiome of a tumor and a healthy mucosa fundamentally differ from each other not only in morphotype and gram staining but also in antagonistic, hemolytic and ribonucleolytic activities. Conclusion. Colonization of the tumor by dominant aggressive Gram-negative bacteria leads to significant changes in the tumor microbiome composition compared with normal mucosa, whose representatives are displaced from the damaged epithelium by more aggressive strains.
dc.relation.ispartofseries Kazan Medical Journal
dc.subject Colorectal cancer
dc.subject Functional activity of bacteria
dc.subject Microbiota
dc.subject Ribonuclease
dc.title Analysis of the intestinal microbiome in colorectal cancer
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 102
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 185
dc.source.id SCOPUS03684814-2021-102-2-SID85105326304


Файлы в этом документе

Данный элемент включен в следующие коллекции

  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

Показать сокращенную информацию

Поиск в электронном архиве


Расширенный поиск

Просмотр

Моя учетная запись

Статистика