dc.contributor.author |
Kabdesh I.M. |
|
dc.contributor.author |
Arkhipova S.S. |
|
dc.contributor.author |
Mukhamedshina Y.O. |
|
dc.contributor.author |
James V. |
|
dc.contributor.author |
Rizvanov A.A. |
|
dc.contributor.author |
Chelyshev Y.A. |
|
dc.date.accessioned |
2022-02-09T20:33:50Z |
|
dc.date.available |
2022-02-09T20:33:50Z |
|
dc.date.issued |
2021 |
|
dc.identifier.issn |
0306-4522 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/169040 |
|
dc.description.abstract |
Emerging evidence supports an increased role for NG2/CSPG4-expressing cells in the process of neuroregeneration and synaptic plasticity, due to the increased production of multifunctional chondroitin sulfate proteoglycan (NG2/CSPG4). However, the response of NG2/CSPG4-expressing cells in spinal cord injury (SCI) remains to be elcudiated. Expression and distribution of NG2/CSPG4-expressing cells were studied by immunoelectron microscopy in the ventral horns (VH) of an intact and injured rat spinal cord. In the intact spinal cord, NG2/CSPG4 expression was detected on the cell membrane and in the cytoplasm of NG2 glia and was absent in neurons. Large amounts of NG2/CSPG4 were found on myelin membranes. The ability of intact astrocytes to produce NG2/CSPG4 was shown, although to a lesser extent than oligodendrocytes and NG2 glia. At 7 days after SCI at the Th8 level in the reactive glial zone of VH, the expression of NG2/CSPG4 sharply increased in NG2 glia at a distance of 3–5 mm and in reactive astrocytes were observed at all investigated distances caudally from the epicenter of injury. The obtained results indicate the presence of NG2/CSPG4-positive astrocytes in the intact spinal cord, and in the case of damage, an increase in the ability of reactive astrocytes to produce NG2/CSPG4. SCI leads to increased expression of NG2/CSPG4 by NG2 glia in the early stages after injury, which decreases with distance from the epicenter of the injury, as well as at later stages. |
|
dc.relation.ispartofseries |
Neuroscience |
|
dc.subject |
ALDH1L1 |
|
dc.subject |
Astrocytes |
|
dc.subject |
electron microscopy |
|
dc.subject |
NG2 glia |
|
dc.subject |
NG2/CSPG4 |
|
dc.subject |
spinal cord injury |
|
dc.title |
The Function of NG2/CSPG4-expressing Cells in the Rat Spinal Cord Injury: An Immunoelectron Microscopy Study |
|
dc.type |
Article |
|
dc.relation.ispartofseries-volume |
467 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
142 |
|
dc.source.id |
SCOPUS03064522-2021-467-SID85107986593 |
|