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Dark-field hyperspectral microscopy for label-free microplastics and nanoplastics detection and identification in vivo: A Caenorhabditis elegans study

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dc.contributor.author Nigamatzyanova L.
dc.contributor.author Fakhrullin R.
dc.date.accessioned 2022-02-09T20:33:31Z
dc.date.available 2022-02-09T20:33:31Z
dc.date.issued 2021
dc.identifier.issn 0269-7491
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/169001
dc.description.abstract Microplastics pollution is a serious ecological threat, severely affecting environments and human health. Tackling microplastics pollution requires an effective methodology to detect minute polymer particles in environmental samples and organisms. Here were report a novel methodology to visualise and identify nanoscale (down to 100 nm) and microscale synthetic commercially-available uniform spherical polymer particles using dark-field hyperspectral microscopy in visible-near infrared (400–1000 nm) wavelength range. Polystyrene particles with diameters between 100 nm–1 μm, polymethacrylate 1 μm and melamine formaldehyde 2 μm microspheres suspended in pure water samples were effectively imaged and chemically identified based on spectral signatures and image-assisted analysis. We succeeded in visualisation and spectral identification of pure and mixed nano- and microplastics in vivo employing optically-transparent Caenorhabditis elegans nematodes as a model to demonstrate the ingestion and tissue distribution of microplastics. As we demonstrate here, dark-field hyperspectral microscopy is capable for differentiating between chemically-different microplastics confined within live invertebrate intestines. Moreover, this optical technology allows for quantitative identification of microplastics ingested by nematodes. We believe that this label-free non-destructive methodology will find numerous applications in environmental nano- and microplastics detection and quantification, investigation of their biodistribution in tissues and organs and nanotoxicology.
dc.relation.ispartofseries Environmental Pollution
dc.subject Caenorhabditis elegans
dc.subject Dark-field hyperspectral microscopy
dc.subject Microplastics
dc.subject Minimum detection size
dc.subject Polystyrene
dc.title Dark-field hyperspectral microscopy for label-free microplastics and nanoplastics detection and identification in vivo: A Caenorhabditis elegans study
dc.type Article
dc.relation.ispartofseries-volume 271
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS02697491-2021-271-SID85098221521


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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