dc.contributor.author |
Makshakova O.N. |
|
dc.contributor.author |
Bogdanova L.R. |
|
dc.contributor.author |
Faizullin D.A. |
|
dc.contributor.author |
Ermakova E.A. |
|
dc.contributor.author |
Zuev Y.F. |
|
dc.contributor.author |
Sedov I.A. |
|
dc.date.accessioned |
2022-02-09T20:32:54Z |
|
dc.date.available |
2022-02-09T20:32:54Z |
|
dc.date.issued |
2021 |
|
dc.identifier.issn |
0144-8617 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/168920 |
|
dc.description.abstract |
The interactions between κ-carrageenan and hen egg-white lysozyme have been studied. In dilute solutions, the insoluble complexes with constant κ-carrageenan/lysozyme ratio of 0.3, or 12 disaccharide units per mole of protein are formed. FTIR-spectroscopy revealed that κ-carrageenan retains its unordered conformation and induces the rise of β-structure in lysozyme. In the complexes formed in concentrated mixtures, κ-carrageenan adopts helical conformation and lysozyme retains its native-like structure. These complexes contain 21 disaccharide units per mole of protein. Molecular modeling showed that flexible coil and rigid double helix of κ-carrageenan have different binding patterns to lysozyme surface. The latter has a strong preference to positively charged spots in lysozyme α-domain while the former also interacts to protein β-domain and stabilizes short-living β-structures. The obtained results confirm the preference of unordered κ-carrageenan to β-structure rich protein regions, which can be further used in the development of carrageenan-based protection of amyloid-like aggregation of proteins. |
|
dc.relation.ispartofseries |
Carbohydrate Polymers |
|
dc.subject |
Computer simulations |
|
dc.subject |
Conformation |
|
dc.subject |
FTIR-spectroscopy |
|
dc.subject |
Lysozyme |
|
dc.subject |
Protein-polysaccharide complexes |
|
dc.subject |
κ-Carrageenan |
|
dc.title |
Interaction-induced structural transformation of lysozyme and kappa-carrageenan in binary complexes |
|
dc.type |
Article |
|
dc.relation.ispartofseries-volume |
252 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.source.id |
SCOPUS01448617-2021-252-SID85092469246 |
|