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dc.contributor.author | Makshakova O.N. | |
dc.contributor.author | Bogdanova L.R. | |
dc.contributor.author | Faizullin D.A. | |
dc.contributor.author | Ermakova E.A. | |
dc.contributor.author | Zuev Y.F. | |
dc.contributor.author | Sedov I.A. | |
dc.date.accessioned | 2022-02-09T20:32:54Z | |
dc.date.available | 2022-02-09T20:32:54Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0144-8617 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/168920 | |
dc.description.abstract | The interactions between κ-carrageenan and hen egg-white lysozyme have been studied. In dilute solutions, the insoluble complexes with constant κ-carrageenan/lysozyme ratio of 0.3, or 12 disaccharide units per mole of protein are formed. FTIR-spectroscopy revealed that κ-carrageenan retains its unordered conformation and induces the rise of β-structure in lysozyme. In the complexes formed in concentrated mixtures, κ-carrageenan adopts helical conformation and lysozyme retains its native-like structure. These complexes contain 21 disaccharide units per mole of protein. Molecular modeling showed that flexible coil and rigid double helix of κ-carrageenan have different binding patterns to lysozyme surface. The latter has a strong preference to positively charged spots in lysozyme α-domain while the former also interacts to protein β-domain and stabilizes short-living β-structures. The obtained results confirm the preference of unordered κ-carrageenan to β-structure rich protein regions, which can be further used in the development of carrageenan-based protection of amyloid-like aggregation of proteins. | |
dc.relation.ispartofseries | Carbohydrate Polymers | |
dc.subject | Computer simulations | |
dc.subject | Conformation | |
dc.subject | FTIR-spectroscopy | |
dc.subject | Lysozyme | |
dc.subject | Protein-polysaccharide complexes | |
dc.subject | κ-Carrageenan | |
dc.title | Interaction-induced structural transformation of lysozyme and kappa-carrageenan in binary complexes | |
dc.type | Article | |
dc.relation.ispartofseries-volume | 252 | |
dc.collection | Публикации сотрудников КФУ | |
dc.source.id | SCOPUS01448617-2021-252-SID85092469246 |