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Cytochalasin B-induced membrane vesicles from human mesenchymal stem cells overexpressing TRAIL, PTEN and IFN-β1 can kill carcinoma cancer cells

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dc.contributor.author Chulpanova D.S.
dc.contributor.author Gilazieva Z.E.
dc.contributor.author Akhmetzyanova E.R.
dc.contributor.author Kletukhina S.K.
dc.contributor.author Rizvanov A.A.
dc.contributor.author Solovyeva V.V.
dc.date.accessioned 2022-02-09T20:32:18Z
dc.date.available 2022-02-09T20:32:18Z
dc.date.issued 2021
dc.identifier.issn 0040-8166
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/168845
dc.description.abstract Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) are of interest as a new vector for the delivery of therapeutic agents into the tumor microenvironment. Cell-free EV-based therapy has a number of advantages over cell-based therapy, since the use of EVs allows avoiding potential undesirable transformation associated with MSCs. MSC-derived EVs can transfer natural proteins with immunomodulatory or antitumor properties. The aim of this study was to produce vesicles from mesenchymal stem cells with simultaneous overexpression of TRAIL, PTEN and IFN-β1 and analyze its antitumor and immunomodulatory properties. In this work, a stable line of human adipose tissue-derived mesenchymal stem cells (hADSCs) with simultaneous overexpression of TRAIL, PTEN and IFN-β1 was produced. To obtain this cell line hADSCs were genetically modified with a genetic multicistronic cassette encoding TRAIL, PTEN, and IFN-β1 genes separated with a self-cleaving P2A peptide nucleotide sequence. Membrane vesicles (CIMVs) were obtained from genetically modified hADSCs using cytochalasin B treatment. Antitumor and immunomodulatory properties of the CIMVs were analyzed in vitro. It was shown that CIMVs isolated from genetically modified hADSCs overexpressing TRAIL, PTEN and IFN-β1 genes are able to activate human immune cells and induce apoptosis in various types of carcinomas in vitro. Thus, the immunomodulatory and antitumor properties of CIMVs were shown. However, further studies on animal models in vivo are required.
dc.relation.ispartofseries Tissue and Cell
dc.subject Cancer therapy
dc.subject Cytochalasin B
dc.subject Extracellular vesicles
dc.subject Immunotherapy
dc.subject Mesenchymal stem cells
dc.title Cytochalasin B-induced membrane vesicles from human mesenchymal stem cells overexpressing TRAIL, PTEN and IFN-β1 can kill carcinoma cancer cells
dc.type Article
dc.relation.ispartofseries-volume 73
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS00408166-2021-73-SID85117167336


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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