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Bioaffinity-based method for catecholamines determination with amperometric DNA sensor

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dc.contributor.author Babkina S.S.
dc.contributor.author Ulakhovich N.A.
dc.contributor.author Medyantseva E.P.
dc.contributor.author Gataulina A.R.
dc.date.accessioned 2021-02-26T20:51:16Z
dc.date.available 2021-02-26T20:51:16Z
dc.date.issued 2020
dc.identifier.issn 2542-064X
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/163247
dc.description.abstract © 2020 Kazan Federal University. All rights reserved. A bioaffinity-based method for the determination of adrenaline and dopamine involving immobilized renatured DNA incorporated into the amperometric biosensor was developed. With the help of spectrophometry and voltammetry data, it was found that the composition of complexes formed with catecholamines and their stability constants were changing with concentration from [КАL2] to [КА2L], where КА – catecholamine, L – base pare, and lg βeff = 10.1–13.5. The high affinity of adrenaline and dopamine to the immobilized renatured DNA molecule was revealed, thereby making it possible to run effective concentration of KA from the analyzed solutions of low concentration including multicomponent fluids. The minimum reporting level was found to be 5.5·10–9 (adrenaline) and 2.0·10–8 (dopamin) mol/L, respectively. The proposed method is characterized by high sensitivity, reproducibility (relative accuracy does not exceed 5%), and single determination time shorter than 30 min.
dc.relation.ispartofseries Uchenye Zapiski Kazanskogo Universiteta. Seriya Estestvennye Nauki
dc.subject Adrenaline
dc.subject Amperometric sensor
dc.subject Bioaffinity-based method
dc.subject Biosensor
dc.subject Catecholamines
dc.subject DNA
dc.subject Dopamine
dc.title Bioaffinity-based method for catecholamines determination with amperometric DNA sensor
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 162
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 5
dc.source.id SCOPUS2542064X-2020-162-1-SID85092891636

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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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