Analyzing the Effectiveness of Adipose Tissue Stem Cell and Microvesicle Therapy in Premature Skin Aging Caused by Chronic Exposure to Ultraviolet Radiation

Abstract

© 2020, Springer Science+Business Media, LLC, part of Springer Nature. Adipose-derived mesenchymal stem cells (ADSCs) and microvesicles (MVs) isolated from ADSCs are promising therapeutic agents for various pathological and physiological skin conditions. Investigate the effects of ADSC and MV therapy on skin regeneration under photoaging conditions in vivo. CD-1 mice were exposed to a course of UV radiation exposure for 6 weeks. After 4 weeks, the animals were injected by multiple intradermal punctures with ADSCs, MVs, or PBS. To analyze the effectiveness of ADSCs and MVs, changes in the microcirculation within the dorsal skin of the mice was assessed using laser Doppler flowmetry. Morphometric and morphological assessment of histological changes were also performed. No differences in skin perfusion were identified at 4 weeks post-injection of ADSCs and MVs. However, histological analysis showed treatment with ADSCs and MVs both led to a decrease in UV-mediated epidermal thickening and improved organization of the dermal layer. ADSC- and ADSC-MV-based therapy acts to prevent skin damage caused by UV photoaging.