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Antimicrobial resistance and genomic characterization of OXA-48-and CTX-M-15-co-producing hypervirulent Klebsiella pneumoniae st23 recovered from nosocomial outbreak

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dc.contributor.author Shaidullina E.
dc.contributor.author Shelenkov A.
dc.contributor.author Yanushevich Y.
dc.contributor.author Mikhaylova Y.
dc.contributor.author Shagin D.
dc.contributor.author Alexandrova I.
dc.contributor.author Ershova O.
dc.contributor.author Akimkin V.
dc.contributor.author Kozlov R.
dc.contributor.author Edelstein M.
dc.date.accessioned 2021-02-25T20:58:07Z
dc.date.available 2021-02-25T20:58:07Z
dc.date.issued 2020
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/162799
dc.description.abstract © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Multidrug resistance (MDR) and hypervirulence (hv) have been long considered distinct evolutionary traits for Klebsiella pneumoniae (Kp), a versatile human pathogen. The recent emergence of Kp strains combining these traits poses a serious global threat. In this article, we describe the phenotypic and genomic characteristics of an MDR hvKp isolate, MAR14-456, representative of a nosocomial outbreak in Moscow, Russia, that was recovered from a postoperative wound in a patient who later developed multiple abscesses, fatal sepsis, and septic shock. Broth microdilution testing revealed decreased susceptibility of MAR14-456 to carbapenems (MICs 0.5–2 mg/L) and a high-level resistance to most β-lactams, β-lactam-β-lactamase-inhibitor combinations, and non-β-lactam antibiotics, except ceftazidime-avibactam, amikacin, tigecycline, and colistin. Whole-genome sequencing using Illumina MiSeq and ONT MinION systems allowed to identify and completely assemble two conjugative resistance plasmids, a typical ‘European’ epidemic IncL/M plasmid that carries the gene of OXA-48 carbapenemase, and an IncFIIK plasmid that carries the gene of CTX-M-15 ESBL and other resistance genes. MLST profile, capsular, lipopolysaccharide, virulence genes encoded on chromosome and IncHI1B/FIB plasmid, and the presence of apparently functional type I-E* CRISPR-Cas system were all characteristic of hvKp ST23, serotype K1-O1v2. Phylogenetic analysis showed the closest relatedness of MAR14-456 to ST23 isolates from China. This report highlights the threat of multiple resistance acquisition by hvKp strain and its spread as a nosocomial pathogen.
dc.subject Hypervirulent ST23
dc.subject Klebsiella pneumoniae
dc.subject Multidrug resistance
dc.subject OXA-48
dc.subject Whole-genome sequencing
dc.title Antimicrobial resistance and genomic characterization of OXA-48-and CTX-M-15-co-producing hypervirulent Klebsiella pneumoniae st23 recovered from nosocomial outbreak
dc.type Article
dc.relation.ispartofseries-issue 12
dc.relation.ispartofseries-volume 9
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1
dc.source.id SCOPUS-2020-9-12-SID85097312461


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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