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Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application

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dc.contributor.author Guseva G.B.
dc.contributor.author Antina E.V.
dc.contributor.author Berezin M.B.
dc.contributor.author Pavelyev R.S.
dc.contributor.author Kayumov A.R.
dc.contributor.author Sharafutdinov I.S.
dc.contributor.author Lisovskaya S.
dc.contributor.author Lodochnikova O.A.
dc.contributor.author Islamov D.R.
dc.contributor.author Usachev K.S.
dc.contributor.author Boichuk S.V.
dc.contributor.author Nikitina L.E.
dc.date.accessioned 2021-02-25T20:36:22Z
dc.date.available 2021-02-25T20:36:22Z
dc.date.issued 2020
dc.identifier.issn 1010-6030
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/162010
dc.description.abstract © 2020 Elsevier B.V. A fluorescent biomarker based on the boron(III) complex with meso-4-methoxycarbonylbutyl-substituted 3,3′,5,5′-tetramethyl-2,2′-dipyrromethene (BODIPY) was synthesized. The BODIPY exhibits a large extinction coefficient (lgε ∼4.82–5.00) at 496–501 nm and high fluorescence quantum yield (∼77–100 %) in the blue-green region of the spectrum (509–518 nm). The maximal fluorescence quantum yield (φ) is observed in non-polar media (∼100 %) while φ slightly decreases to ∼90 % in alcohols (with the exception of octanol-1) and to ∼77 % in electron-donating dimethyl sulfoxide (DMSO). The BODIPY fluorophore demonstrates high photostability with the half-life of 41.4 and 91 h in toluene and cyclohexane, respectively. The proposed luminophore preferentially stains gram-positive bacteria and can be used for differential staining of gram-positive and gram-negative bacteria in mixed cultures. BODIPY also accumulates in the cytoplasm of the mammalian cells giving polar micro-speckled staining pattern which is more intensive in the tumor cells when compared to the fibroblasts. The pronounced affinity of BODIPY to mitochondria of eukaryotic cells could be used for specific staining of these organelles.
dc.relation.ispartofseries Journal of Photochemistry and Photobiology A: Chemistry
dc.subject Biomarker
dc.subject BODIPY
dc.subject Fluorescent dyes
dc.subject Photostability
dc.subject Spectral properties
dc.title Meso-substituted-BODIPY based fluorescent biomarker: Spectral characteristics, photostability and possibilities for practical application
dc.type Article
dc.relation.ispartofseries-volume 401
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS10106030-2020-401-SID85088512805

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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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