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An Improved Substrate for Superior Imaging of Individual Biomacromolecules with Atomic Force Microscopy

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dc.contributor.author Klinov D.V.
dc.contributor.author Protopopova A.D.
dc.contributor.author Andrianov D.S.
dc.contributor.author Litvinov R.I.
dc.contributor.author Weisel J.W.
dc.date.accessioned 2021-02-25T20:35:39Z
dc.date.available 2021-02-25T20:35:39Z
dc.date.issued 2020
dc.identifier.issn 0927-7765
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/161923
dc.description.abstract © 2020 Elsevier B.V. High-resolution atomic force microscopy (AFM) of biomacromolecules is a valuable method for structural studies in biology. Traditionally, the surfaces used for AFM imaging of individual molecules are limited to mica, graphite, and glass. Because these substrates have certain shortcomings, new or modified surfaces that improve the quality of AFM imaging are highly desirable. Here, we describe an improved substrate for imaging of individual biomacromolecules with high-resolution AFM based on graphite surfaces coated by physical adsorption. We provide a detailed methodology, including the chemical structure, synthesis, characterization and the use of a substance that modifies the surface of freshly cleaved graphite, making it suitable for adsorption and AFM visualization of various biomacromolecules while minimizing spatial distortions. We illustrate the advantages of the modified graphite over regular surfaces with examples of high-resolution single-molecule imaging of proteins, polysaccharides, DNA and DNA-protein complexes. The proposed methodology is easy to use and helps to improve substantially AFM imaging of biomacromolecules of various natures, including flexible and/or unstructured sub-molecular regions that are not seen on other AFM substrates. The proposed technique has the potential to improve the use of AFM in structural biology for visualization and morphometric characterization of macromolecular objects.
dc.relation.ispartofseries Colloids and Surfaces B: Biointerfaces
dc.subject Atomic force microscopy
dc.subject Graphite modification
dc.subject Highly oriented pyrolytic graphite
dc.subject Nucleic acids
dc.subject Polysaccharides
dc.subject Proteins
dc.title An Improved Substrate for Superior Imaging of Individual Biomacromolecules with Atomic Force Microscopy
dc.type Article
dc.relation.ispartofseries-volume 196
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS09277765-2020-196-SID85089679838

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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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