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Impact of oppositely charged shell and cores on interaction of core-shell colloids with differently charged proteins as a route for tuning of the colloids cytotoxicity

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dc.contributor.author Elistratova J.
dc.contributor.author Faizullin B.
dc.contributor.author Strelnik I.
dc.contributor.author Gerasimova T.
dc.contributor.author Khairullin R.
dc.contributor.author Sapunova A.
dc.contributor.author Voloshina A.
dc.contributor.author Mukhametzyanov T.
dc.contributor.author Musina E.
dc.contributor.author Karasik A.
dc.contributor.author Mustafina A.
dc.date.accessioned 2021-02-25T20:35:38Z
dc.date.available 2021-02-25T20:35:38Z
dc.date.issued 2020
dc.identifier.issn 0927-7765
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/161922
dc.description.abstract © 2020 Elsevier B.V. The present work represents interactions between the core-shell nanoparticles and different proteins, exemplified by lysozyme (LSZ), pepsin, bovine serum albumin (BSA), thioredoxin (TRX) and yellow fluorescent protein (YFP). The core-shell morphology derives from the non-covalent deposition of polyethyleneimine (PEI) onto nanoprecipitated luminescent complex (AuCl)2L (L is cyclic PNNP ligand). Analysis of the data obtained by DLS, CD spectroscopy, luminescence derived from both (AuCl)2L and YFP reveal the electrostatically driven interaction of negatively charged proteins with the shell of PEI-(AuCl)2L. The fluorescence of YFP enables to reveal the inclusion of the protein molecules into the shell. The lack of any luminescent response of PEI-(AuCl)2L on TRX conforms its electrostatically driven interactions with the shell which, in turn, excludes a binding of the exposed thiol moieties with (AuCl)2L. The negatively charged surface of pepsin provides the greatest recharging of the PEI-based shell versus the other proteins, which is followed by the enhanced luminescence of (AuCl)2L. The significant effect of PEI-(AuCl)2L on the CD spectra of LSZ followed by the decreased intensity of (AuCl)2L-based luminescence points to specific interaction mode of PEI-(AuCl)2L with LSZ. The flow cytometry and fluorescent microscopy measurements revealed efficient internalization of PEI-(AuCl)2L into the Wi-38 cell samples resulting in the efficient staining of all cell organelles. The concentration dependent cytotoxicity of PEI-(AuCl)2L is detectably enhanced by LSZ, which is correlated with its interaction mode with the nanoparticles.
dc.relation.ispartofseries Colloids and Surfaces B: Biointerfaces
dc.subject Au(I) complex
dc.subject Cell internalization
dc.subject Core-shell nanoparticles
dc.subject Cytotoxicity
dc.subject Luminescence
dc.subject Proteins
dc.title Impact of oppositely charged shell and cores on interaction of core-shell colloids with differently charged proteins as a route for tuning of the colloids cytotoxicity
dc.type Article
dc.relation.ispartofseries-volume 196
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS09277765-2020-196-SID85089430637


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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