Pillar[5]arenes as potential personage for DNA compactization and gene therapy

Abstract

© 2020 Elsevier B.V. Here we demonstrated that pillar[5]arenes with counterions I− and Cl− show the ability to plasmid compactization and increasing bacterial transformation efficiently. Pillar[5]arenes have been tested for binding with palindromic decamer oligonucleotide and interacting with plasmid DNA. The complexation of pillar[5]arene with oligonucleotide has been shown by NMR- and CD-spectroscopy. Pillar[5]arenes form complexes with oligonucleotide in solution in the 1:1 or 1:2 stoichiometry. Molecular modeling allowed to constructs the models of these complexes. Pillar[5]arene interaction with the plasmid DNA have been studied using atomic force microscopy. According to AFM images pillar[5]arene-I− and pillar[5]arene-Cl− packed up the plasmid DNA to aggregates with diameter about 100 nm with different morphology. An increase in DNA transformation efficiency into bacterial cells has been shown for pillar[5]arenes with counterions I− and Cl−.