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Pillar[5]arenes as potential personage for DNA compactization and gene therapy

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dc.contributor.author Skvortsova P.V.
dc.contributor.author Faizullin D.A.
dc.contributor.author Ermakova E.A.
dc.contributor.author Shurpik D.N.
dc.contributor.author Gogoleva N.E.
dc.contributor.author Gogolev Y.V.
dc.contributor.author Ziganshina S.A.
dc.contributor.author Stoikov I.I.
dc.contributor.author Zuev Y.F.
dc.contributor.author Khairutdinov B.I.
dc.date.accessioned 2021-02-25T20:34:03Z
dc.date.available 2021-02-25T20:34:03Z
dc.date.issued 2020
dc.identifier.issn 0167-7322
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/161769
dc.description.abstract © 2020 Elsevier B.V. Here we demonstrated that pillar[5]arenes with counterions I− and Cl− show the ability to plasmid compactization and increasing bacterial transformation efficiently. Pillar[5]arenes have been tested for binding with palindromic decamer oligonucleotide and interacting with plasmid DNA. The complexation of pillar[5]arene with oligonucleotide has been shown by NMR- and CD-spectroscopy. Pillar[5]arenes form complexes with oligonucleotide in solution in the 1:1 or 1:2 stoichiometry. Molecular modeling allowed to constructs the models of these complexes. Pillar[5]arene interaction with the plasmid DNA have been studied using atomic force microscopy. According to AFM images pillar[5]arene-I− and pillar[5]arene-Cl− packed up the plasmid DNA to aggregates with diameter about 100 nm with different morphology. An increase in DNA transformation efficiency into bacterial cells has been shown for pillar[5]arenes with counterions I− and Cl−.
dc.relation.ispartofseries Journal of Molecular Liquids
dc.subject Bacterial transformation
dc.subject Pillar[n]arene
dc.subject Plasmid compactization
dc.title Pillar[5]arenes as potential personage for DNA compactization and gene therapy
dc.type Article
dc.relation.ispartofseries-volume 319
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS01677322-2020-319-SID85092470078


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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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