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The Contribution of L-Type Calcium Channels to Acetylcholine Secretion in Frog and Mouse Neuromuscular Junctions with Active and Inactivated Voltage-Gated Potassium Channels

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dc.contributor.author Tsentsevitsky A.N.
dc.contributor.author Khuzakhmetova V.F.
dc.contributor.author Khaziev E.F.
dc.contributor.author Kovyazina I.V.
dc.date.accessioned 2021-02-25T20:33:49Z
dc.date.available 2021-02-25T20:33:49Z
dc.date.issued 2020
dc.identifier.issn 0097-0549
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/161739
dc.description.abstract © 2020, Springer Science+Business Media, LLC, part of Springer Nature. We report here experiments addressing the contribution of L-type Ca2+ channels to evoked acetylcholine secretion from frog and mouse motor nerve endings with active and inactivated voltage-gated K+ channels. These studies evaluated the effects of the specific L-type Ca2+ channel blocker nitrendipine on the quantum composition of endplate currents and the time course of the secretion of acetylcholine quanta in intact preparations and after preliminary blockade of voltage-gated K+ channels with 4-aminopyridine (4-AP) in medium with depressed and physiological Ca2+ levels. A fluorescence method was used to measure calcium transients reflecting the integral influx of Ca2+ into nerve endings; computer modeling was applied to the processes underlying exocytosis in the presence of the two types of Ca2+ channel (N and L) and with different durations of nerve ending action potentials. In frog synapses, L-type Ca2+ channels were found to contribute to evoked acetylcholine secretion in the presence of active K+ channels, but only in the presence of a depressed Ca2+ level in the medium; on inactivation of voltage-gated K+ channels, the contribution of L-type channels to the secretory process became less significant. At a physiological Ca2+ level, the involvement of L-type channels in evoked acetylcholine secretion was apparent, as in mouse synapses, only in conditions of blockade of voltage-gated K+ channels.
dc.relation.ispartofseries Neuroscience and Behavioral Physiology
dc.subject acetylcholine
dc.subject calcium channel
dc.subject neuromuscular synapse
dc.subject potassium channel
dc.title The Contribution of L-Type Calcium Channels to Acetylcholine Secretion in Frog and Mouse Neuromuscular Junctions with Active and Inactivated Voltage-Gated Potassium Channels
dc.type Article
dc.relation.ispartofseries-issue 7
dc.relation.ispartofseries-volume 50
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 920
dc.source.id SCOPUS00970549-2020-50-7-SID85092233961


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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