dc.contributor.author |
Tsentsevitsky A.N. |
|
dc.contributor.author |
Khuzakhmetova V.F. |
|
dc.contributor.author |
Khaziev E.F. |
|
dc.contributor.author |
Kovyazina I.V. |
|
dc.date.accessioned |
2021-02-25T20:33:49Z |
|
dc.date.available |
2021-02-25T20:33:49Z |
|
dc.date.issued |
2020 |
|
dc.identifier.issn |
0097-0549 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/161739 |
|
dc.description.abstract |
© 2020, Springer Science+Business Media, LLC, part of Springer Nature. We report here experiments addressing the contribution of L-type Ca2+ channels to evoked acetylcholine secretion from frog and mouse motor nerve endings with active and inactivated voltage-gated K+ channels. These studies evaluated the effects of the specific L-type Ca2+ channel blocker nitrendipine on the quantum composition of endplate currents and the time course of the secretion of acetylcholine quanta in intact preparations and after preliminary blockade of voltage-gated K+ channels with 4-aminopyridine (4-AP) in medium with depressed and physiological Ca2+ levels. A fluorescence method was used to measure calcium transients reflecting the integral influx of Ca2+ into nerve endings; computer modeling was applied to the processes underlying exocytosis in the presence of the two types of Ca2+ channel (N and L) and with different durations of nerve ending action potentials. In frog synapses, L-type Ca2+ channels were found to contribute to evoked acetylcholine secretion in the presence of active K+ channels, but only in the presence of a depressed Ca2+ level in the medium; on inactivation of voltage-gated K+ channels, the contribution of L-type channels to the secretory process became less significant. At a physiological Ca2+ level, the involvement of L-type channels in evoked acetylcholine secretion was apparent, as in mouse synapses, only in conditions of blockade of voltage-gated K+ channels. |
|
dc.relation.ispartofseries |
Neuroscience and Behavioral Physiology |
|
dc.subject |
acetylcholine |
|
dc.subject |
calcium channel |
|
dc.subject |
neuromuscular synapse |
|
dc.subject |
potassium channel |
|
dc.title |
The Contribution of L-Type Calcium Channels to Acetylcholine Secretion in Frog and Mouse Neuromuscular Junctions with Active and Inactivated Voltage-Gated Potassium Channels |
|
dc.type |
Article |
|
dc.relation.ispartofseries-issue |
7 |
|
dc.relation.ispartofseries-volume |
50 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
920 |
|
dc.source.id |
SCOPUS00970549-2020-50-7-SID85092233961 |
|