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dc.contributor.author | Serebriiskii I.G. | |
dc.contributor.author | Elmekawy M. | |
dc.contributor.author | Golemis E.A. | |
dc.date.accessioned | 2021-02-25T06:47:16Z | |
dc.date.available | 2021-02-25T06:47:16Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/161016 | |
dc.description.abstract | © Springer Science+Business Media, LLC, part of Springer Nature 2020. Cerebral cavernous malformation (CCM) is a vascular malformation of the central nervous system that is associated with leaky capillaries, and a predisposition to serious clinical conditions including intracerebral hemorrhage and seizures. Germline or sporadic mutations in the CCM1/KRIT1 gene are responsible for the majority of cases of CCM. In this article, we describe the original characterization of the CCM1/KRIT1 gene. This cloning was done through the use of a variant of the yeast two-hybrid screen known as the interaction trap, using the RAS-family GTPase KREV1/RAP1A as a bait. The partial clone of KRIT1 (Krev1 Interaction Trapped) initially identified was extended through 5′RACE and computational analysis to obtain a full-length cDNA, then used in a sequential screen to define the integrin-associated ICAP1 protein as a KRIT1 partner protein. We discuss how these interactions are relevant to the current understanding of KRIT1/CCM1 biology, and provide a protocol for library screening with the Interaction Trap. | |
dc.relation.ispartofseries | Methods in Molecular Biology | |
dc.subject | CCM1 | |
dc.subject | Cerebral cavernous malformations | |
dc.subject | ICAP1 | |
dc.subject | Interaction trap | |
dc.subject | KREV1 | |
dc.subject | KRIT1 | |
dc.subject | RAP1A | |
dc.subject | Yeast two-hybrid | |
dc.title | Identification of the KRIT1 protein by LexA-based yeast two-hybrid system | |
dc.type | Chapter | |
dc.relation.ispartofseries-volume | 2152 | |
dc.collection | Публикации сотрудников КФУ | |
dc.relation.startpage | 269 | |
dc.source.id | SCOPUS10643745-2020-2152-SID85086354775 |