dc.contributor.author |
Serebriiskii I.G. |
|
dc.contributor.author |
Elmekawy M. |
|
dc.contributor.author |
Golemis E.A. |
|
dc.date.accessioned |
2021-02-25T06:47:16Z |
|
dc.date.available |
2021-02-25T06:47:16Z |
|
dc.date.issued |
2020 |
|
dc.identifier.issn |
1064-3745 |
|
dc.identifier.uri |
https://dspace.kpfu.ru/xmlui/handle/net/161016 |
|
dc.description.abstract |
© Springer Science+Business Media, LLC, part of Springer Nature 2020. Cerebral cavernous malformation (CCM) is a vascular malformation of the central nervous system that is associated with leaky capillaries, and a predisposition to serious clinical conditions including intracerebral hemorrhage and seizures. Germline or sporadic mutations in the CCM1/KRIT1 gene are responsible for the majority of cases of CCM. In this article, we describe the original characterization of the CCM1/KRIT1 gene. This cloning was done through the use of a variant of the yeast two-hybrid screen known as the interaction trap, using the RAS-family GTPase KREV1/RAP1A as a bait. The partial clone of KRIT1 (Krev1 Interaction Trapped) initially identified was extended through 5′RACE and computational analysis to obtain a full-length cDNA, then used in a sequential screen to define the integrin-associated ICAP1 protein as a KRIT1 partner protein. We discuss how these interactions are relevant to the current understanding of KRIT1/CCM1 biology, and provide a protocol for library screening with the Interaction Trap. |
|
dc.relation.ispartofseries |
Methods in Molecular Biology |
|
dc.subject |
CCM1 |
|
dc.subject |
Cerebral cavernous malformations |
|
dc.subject |
ICAP1 |
|
dc.subject |
Interaction trap |
|
dc.subject |
KREV1 |
|
dc.subject |
KRIT1 |
|
dc.subject |
RAP1A |
|
dc.subject |
Yeast two-hybrid |
|
dc.title |
Identification of the KRIT1 protein by LexA-based yeast two-hybrid system |
|
dc.type |
Chapter |
|
dc.relation.ispartofseries-volume |
2152 |
|
dc.collection |
Публикации сотрудников КФУ |
|
dc.relation.startpage |
269 |
|
dc.source.id |
SCOPUS10643745-2020-2152-SID85086354775 |
|