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Data on proteomic profiling of cells and extracellular vesicles of the melittin-resistant Acholeplasma laidlawii strain

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dc.contributor.author Medvedeva E.
dc.contributor.author Mouzykantov A.
dc.contributor.author Baranova N.
dc.contributor.author Dramchini M.
dc.contributor.author Chernova O.
dc.contributor.author Chernov V.
dc.date.accessioned 2020-01-21T20:57:37Z
dc.date.available 2020-01-21T20:57:37Z
dc.date.issued 2019
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/157871
dc.description.abstract © 2019 The Authors Acholeplasma laidlawii (class Mollicutes), a major contaminant of cell cultures, quickly adapts to various classes of antimicrobials, including antimicrobial peptides. The extracellular vesicles of this bacterium can play a significant role in the development of drug-resistance Chernov et al., 2018. We compared the cellular and vesicular proteomes of A. laidlawii strains with differing susceptibility to melittin (an antimicrobial peptide from bee venom), the genomes of which we have previously sequenced. We extracted soluble proteins from cells and extracellular vesicles of the A. laidlawii PG8RMel strain showing an increased resistance to melittin, and compared them with the cellular proteome and a previously obtained vesicular proteome of the original (reference) A. laidlawii PG8B strain Chernov et al., 2014. The cellular proteome profile of the A. laidlawii strains differing in susceptibility to melittin was determined by using two-dimensional gel electrophoresis and MALDI-TOF/TOF MS. Here we present the cellular proteins that were differentially expressed. The vesicular proteome profile was determined by using one-dimensional electrophoresis and chromatography-mass spectrometry. A list of the extracellular vesicles proteins of the melittin-resistant A. laidlawii strain is presented here.
dc.subject 2DE
dc.subject Acholeplasma laidlawii
dc.subject Extracellular vesicles
dc.subject LC–ESI-MS/MS
dc.subject MALDI-TOF/TOF MS
dc.subject Melittin
dc.subject Proteome
dc.subject Susceptibility
dc.title Data on proteomic profiling of cells and extracellular vesicles of the melittin-resistant Acholeplasma laidlawii strain
dc.relation.ispartofseries-volume 25
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2019-25-SID85076506024


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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