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Probing surface membrane receptors using engineered bacteriophage bioconjugates

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dc.contributor.author Lomakin Y.
dc.contributor.author Kaminskaya A.
dc.contributor.author Stepanov A.
dc.contributor.author Shmidt A.
dc.contributor.author Gabibov A.
dc.contributor.author Belogurov A.
dc.date.accessioned 2020-01-21T20:45:58Z
dc.date.available 2020-01-21T20:45:58Z
dc.date.issued 2019
dc.identifier.issn 1043-1802
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/157751
dc.description.abstract © 2019 American Chemical Society. Specific recognition of ligands by surface receptors of eukaryotic cells is a fundamental process in sensing of the exogenous environment, including cell-to-cell communication. These interactions are therefore widely probed in both basic studies and drug development to enhance or interrupt them. Here, we designed a high-throughput publicly available platform for visualization and selection of eukaryotic cells according to the specificity of surface-exposed receptors by consolidation of phage display and flow cytometry techniques. Polypeptide ligands for membrane receptors are incorporated into every copy of p3 protein of M13K07 bacteriophage, which is intracellularly biotinylated to further accept PE-Cy7-labled streptavidin. Transgenic antigen-specific B-cells expressing membrane-tethered lymphoid B-cell receptor in a single-chain format interacted with engineered bacteriophages exposing the polypeptide ligand with an unprecedented selectivity of 97% and a false-positive detection value of 2.0%. Multivalent binding of the phage bioconjugates with the receptor provided significantly better specificity and sensitivity allowing application of engineered bacteriophage bioconjugates at a concentration 3 orders of magnitude lower in comparison with synthetic biotinylated peptide. We suggest that the platform described in this work may be applied either for routine staining or characterization of orphan membrane receptors exposed on the surface of living mammalian cells in their native environment.
dc.relation.ispartofseries Bioconjugate Chemistry
dc.title Probing surface membrane receptors using engineered bacteriophage bioconjugates
dc.type Article
dc.relation.ispartofseries-issue 5
dc.relation.ispartofseries-volume 30
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1500
dc.source.id SCOPUS10431802-2019-30-5-SID85065784792

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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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