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Oleuropein modulates glioblastoma miRNA pattern different from Olea europaea leaf extract

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dc.contributor.author Tezcan G.
dc.contributor.author Aksoy S.
dc.contributor.author Tunca B.
dc.contributor.author Bekar A.
dc.contributor.author Mutlu M.
dc.contributor.author Cecener G.
dc.contributor.author Egeli U.
dc.contributor.author Kocaeli H.
dc.contributor.author Demirci H.
dc.contributor.author Taskapilioglu M.
dc.date.accessioned 2020-01-21T20:40:37Z
dc.date.available 2020-01-21T20:40:37Z
dc.date.issued 2019
dc.identifier.issn 0960-3271
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/157592
dc.description.abstract © The Author(s) 2019. Glioblastoma (GBM) is the most prevalent and deadliest subtype of glioma. Despite current innovations in existing therapeutic modalities, GBM remains incurable, and alternative therapies are required. Previously, we demonstrated that Olea europaea leaf extract (OLE) kills GBM cells by modulating miR-181b, miR-137, miR-153 and Let-7d expression. However, although oleuropein (OL) is the main compound in OLE, its role in the antitumour effect of OLE remains unknown. This study determined the effect of OL on GBM cell line T98G and compared the results with our previous findings regarding the effect of OLE on the same cell line. The antiproliferative activity of OL and its effect on temozolomide (TMZ) response were tested inT98G cells using WST-1 assay. OL inhibition was evaluated using one-way analysis of variance with Tukey’s post hoc test. The effect of OL on miR-181b, miR-137, miR-153 and Let-7d expression was assessed using quantitative reverse transcription polymerase chain reaction. Fold differences in expression between untreated, OL or OL + TMZ-treated samples were calculated using 2−ΔCt method. Significance was evaluated using an independent sample t-test. Treatment with 277.5 and 555 µM OL resulted in 39.51% and 75.40% reductions in T98G cells within 24 h. Coadministration of 325 µM TMZ and 277.5 or 555 µM, OL caused 2.08- and 2.83-fold increases, respectively, in the therapeutic effect of TMZ. OL + TMZ significantly increased microRNA expression, particularly Let-7d, than OLE. In conclusion, OL has an antitumour effect on GBM cells mainly via regulation of Let-7d expression. The present results also indicate other minor compounds in OLE play important anticancer roles.
dc.relation.ispartofseries Human and Experimental Toxicology
dc.subject Glioblastoma multiforme
dc.subject Olea europaea
dc.subject oleuropein
dc.subject temozolomide
dc.title Oleuropein modulates glioblastoma miRNA pattern different from Olea europaea leaf extract
dc.type Article
dc.relation.ispartofseries-issue 9
dc.relation.ispartofseries-volume 38
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1102
dc.source.id SCOPUS09603271-2019-38-9-SID85067832222


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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