Genes polymorphism of innate immunity in children with neonatal sepsis

Abstract

© 2019, Pediatria Ltd. All rights reserved. Objective of the research - to identify polymorphism of genes encoding innate immunity molecules in children with neonatal sepsis (NS). Materials and methods: 70 newborns were examined. They were divided into 2 groups. The first group consisted of 44 children with NS, 17 of which (39%) were born prematurely. The main foci of NS were: Pneumonia (13 cases, 29%), meningitis (12 children, 27%), enterocolitis (10 cases, 23%), pneumonia in combination with enterocolitis (2 children, 5%) and pemphigus (2 children, 5%). In 5 children (10%) the disease was in the form of septicemia. 2nd, the control group consisted of 26 children who had no signs of an infectious disease, 11 of whom were born prematurely. Results: The risk of NS developing is associated with the mutant genotype G/A in TLR4 Asp299Gly polymorphism (OR=9,4; p=0,03) and mutations in the coding and regulatory parts of the IL1β gene, in particular with the CT and TT genotypes of the IL3β gene polymorphism (OR=3,6; p=0,03). For the TLR4 Asp299Gly gene polymorphism, the frequency of the mutant Gly allele in the control group was 1,9%, in the group of children with NS - 13,6% (p=0,03). For the IL1β C3953T gene, the mutation frequency of the T allele in the control group was 11,5%, in children with sepsis - 28,4% (p=0,02). The proportion of hetero- and homozygotes for this allele in the group of children with NA was 48 and 4%, respectively. Conclusion: Mutant genotypes of the TLR4 gene for Asp299Gly polymorphism and proinflammatory cytokine IL1β for C3953T polymorphism are associated with a high risk of developing NS.