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Mesenchymal stem cell therapy for spinal cord contusion: A comparative study on small and large animal models

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dc.contributor.author Mukhamedshina Y.
dc.contributor.author Shulman I.
dc.contributor.author Ogurcov S.
dc.contributor.author Kostennikov A.
dc.contributor.author Zakirova E.
dc.contributor.author Akhmetzyanova E.
dc.contributor.author Rogozhin A.
dc.contributor.author Masgutova G.
dc.contributor.author James V.
dc.contributor.author Masgutov R.
dc.contributor.author Lavrov I.
dc.contributor.author Rizvanov A.
dc.date.accessioned 2020-01-15T22:10:14Z
dc.date.available 2020-01-15T22:10:14Z
dc.date.issued 2019
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/156832
dc.description.abstract © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Here, we provide a first comparative study of the therapeutic potential of allogeneic mesenchymal stem cells derived from bone marrow (BM-MSCs), adipose tissue (AD-MSCs), and dental pulp (DP-MSCs) embedded in fibrin matrix, in small (rat) and large (pig) spinal cord injury (SCI) models during subacute period of spinal contusion. Results of behavioral, electrophysiological, and histological assessment as well as immunohistochemistry and real-time polymerase chain reaction analysis suggest that application of AD-MSCs combined with a fibrin matrix within the subacute period in rats (2 weeks after injury), provides significantly higher post-traumatic regeneration compared to a similar application of BM-MSCs or DP-MSCs. Within the rat model, use of AD-MSCs resulted in a marked change in: (1) restoration of locomotor activity and conduction along spinal axons; (2) reduction of post-traumatic cavitation and enhancing tissue retention; and (3) modulation of microglial and astroglial activation. The effect of an autologous application of AD-MSCs during the subacute period after spinal contusion was also confirmed in pigs (6 weeks after injury). Effects included: (1) partial restoration of the somatosensory spinal pathways; (2) reduction of post-traumatic cavitation and enhancing tissue retention; and (3) modulation of astroglial activation in dorsal root entry zone. However, pigs only partially replicated the findings observed in rats. Together, these results indicate application of AD-MSCs embedded in fibrin matrix at the site of SCI during the subacute period can facilitate regeneration of nervous tissue in rats and pigs. These results, for the first time, provide robust support for the use of AD-MSC to treat subacute SCI.
dc.subject Adipose tissue
dc.subject Bone marrow
dc.subject Dental pulp
dc.subject Fibrin matrix
dc.subject Mesenchymal stem cells
dc.subject Pig
dc.subject Rat
dc.subject Spinal cord injury
dc.title Mesenchymal stem cell therapy for spinal cord contusion: A comparative study on small and large animal models
dc.type Article
dc.relation.ispartofseries-issue 12
dc.relation.ispartofseries-volume 9
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS-2019-9-12-SID85076265550


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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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