The monitoring of sredniy kaban lake by 16s rrna gene amplicon data set-based bacterial diversity

Abstract

© 2019, Dorma Journals. All rights reserved. The paper presents the results of research of bacterial diversity of Sredniy Kaban Lake (Kazan, Russia) for 2016-2017, using the marker gene 16S rRNA of aquatic organisms, based on the method of next-generation sequencing. Sredniy Kaban, Verhniy Kaban and Nizhniy Kaban are included in the system of Kaban Lakes. They are located in the center of a large industrial city, and are exposed to anthropogenic load. According to ecological studies, Kaban Lakes are polluted. The sequences of 16S rRNA Bacteria gene fragment of the freshwater lake Sredniy Kaban were submitted to the international database in fastq format on the website NCBI with the numbers SRR7510929 (2016) and SRR7516240 (2017). The comparative analysis of metagenomic data showed a significant change in bacterial diversity over the years. A total of 98822 (2016) and 26046 (2017) high-quality reads were obtained; 67% (2016) and 54% (2017) of the bacterial population were classified to the genus level, while 3% (2017) was classified to the species level. In total, 15 species of Bacteria were identified. Among them, the dangerous bacteria Acinetobacter, occurring universally, were found the most often. This type of bacteria can pose a threat to human health. Therefore, the species composition of Bacteria community should be taken into account when assessing the ecological state of water reservoirs.