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dc.contributor.author | Zakirova E. | |
dc.contributor.author | Shalimov D. | |
dc.contributor.author | Garanina E. | |
dc.contributor.author | Zhuravleva M. | |
dc.contributor.author | Rutland C. | |
dc.contributor.author | Rizvanov A. | |
dc.date.accessioned | 2020-01-15T22:07:40Z | |
dc.date.available | 2020-01-15T22:07:40Z | |
dc.date.issued | 2019 | |
dc.identifier.uri | https://dspace.kpfu.ru/xmlui/handle/net/156666 | |
dc.description.abstract | © 2019 Zakirova, Shalimov, Garanina, Zhuravleva, Rutland and Rizvanov. Objectives: Large full-thickness skin defects represent a serious veterinary problem. Methods: We have developed novel bioactive 3D-matrixes based on fibrin glue Tissucol (Baxter), containing the combination of the adenoviral constructs with genes vascular endothelial growth factor 165 (VEGF165) and fibroblast growth factor two (FGF2; construct Ad5-VEGF165 + Ad5-FGF2) or multipotent mesenchymal stem cells, genetically modified with these constructs. Results: In vitro studies confirmed the biosynthesis of VEGF165 and FGF2 mRNA in the transduced cells. Ad5-VEGF165 + Ad5-FGF2- transduced multipotent mesenchymal stem cells showed an enhanced capacity to form capillary-like tubes in vitro. Bioactive 3D-matrix application enhanced granulation tissue formation and epithelialization of non-healing, large bite wounds in a dog. Successful wound healing was observed with a positive clinical outcome for the canine patient. This research and application of regenerative gene therapy alongside a novel bioactive 3D-matrix shows promising clinical applications for the future in both dogs and other mammals including humans. | |
dc.subject | 3D matrix | |
dc.subject | FGF2 | |
dc.subject | Fibrin glue | |
dc.subject | Mesenchymal stem cells | |
dc.subject | VEGF165 | |
dc.title | Use of biologically active 3D matrix for extensive skin defect treatment in veterinary practice: Case report | |
dc.type | Article | |
dc.relation.ispartofseries-issue | MAR | |
dc.relation.ispartofseries-volume | 6 | |
dc.collection | Публикации сотрудников КФУ | |
dc.source.id | SCOPUS-2019-6--SID85065392279 |