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Depression of the Evoked Quantal Acetylcholine Secretion in Frog Neuromuscular Junction by Phospholipases A2 from the Venom of Steppe Viper Vipera ursinii renardi

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dc.contributor.author Kovyazina I.
dc.contributor.author Kopylova N.
dc.contributor.author Utkin Y.
dc.contributor.author Bukharaeva E.
dc.contributor.author Nikolsky E.
dc.contributor.author Vulfius C.
dc.date.accessioned 2020-01-15T21:59:10Z
dc.date.available 2020-01-15T21:59:10Z
dc.date.issued 2019
dc.identifier.issn 1990-7478
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/156462
dc.description.abstract © 2019, Pleiades Publishing, Ltd. Abstract: In this work we studied the influence of phospholipase A2 vurtoxin and its homologue lacking enzymatic activity (Vur-S49), isolated from the venom of steppe viper Vipera ursinii renardi, on the amplitude and temporal parameters of spontaneous and evoked endplate currents (EPCs) in the neuromuscular junction of frog Rana ridibunda. The experiments showed that both vurtoxin and Vur-S49 reduce the EPC quantal content. The amplitude and time course of spontaneous (one-quantal) signals remained unchanged, suggesting that these proteins do not block nicotinic acetylcholine receptors (nAChRs) on the postsynaptic membrane. Depressing effect in the presence of enzymatically inactive Vur-S49 suggested that the decrease in the EPC quantal content in the presence of these proteins cannot be explained exclusively by phospholipolytic activity manifested by vurtoxin. On the basis of our previous data we suggested an interaction of the proteins studied with presynaptic α7 nAChRs. Selective antagonist of α7 nicotinic receptors methyllycaconitine (MLA) reduced the EPC quantal content as well. Depressing action of MLA on the evoked secretion of acetylcholine implies the involvement of the presynaptic α7 nAChRs in the regulation of the evoked quantal secretion in the frog neuromuscular junction. However, in the presence of MLA the effects of vurtoxin and Vur-S49 on the EPC quantal content in the nerve-muscle preparation remained unchanged. The data obtained suggest that presynaptic effects of the proteins studied are not directed at α7 nAChRs but could be mediated by interaction with some other synaptic targets.
dc.relation.ispartofseries Biochemistry (Moscow) Supplement Series A: Membrane and Cell Biology
dc.subject endplate current
dc.subject neuromuscular junction
dc.subject nicotinic acetylcholine receptor
dc.subject phospholipase
dc.subject viper venom
dc.title Depression of the Evoked Quantal Acetylcholine Secretion in Frog Neuromuscular Junction by Phospholipases A2 from the Venom of Steppe Viper Vipera ursinii renardi
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 13
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 78
dc.source.id SCOPUS19907478-2019-13-1-SID85064574990


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    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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