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Quantitative changes in perineuronal nets in development and posttraumatic condition

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dc.contributor.author Lipachev N.
dc.contributor.author Arnst N.
dc.contributor.author Melnikova A.
dc.contributor.author Jäälinoja H.
dc.contributor.author Kochneva A.
dc.contributor.author Zhigalov A.
dc.contributor.author Kulesskaya N.
dc.contributor.author Aganov A.
dc.contributor.author Mavlikeev M.
dc.contributor.author Rauvala H.
dc.contributor.author Kiyasov A.
dc.contributor.author Paveliev M.
dc.date.accessioned 2020-01-15T21:48:03Z
dc.date.available 2020-01-15T21:48:03Z
dc.date.issued 2019
dc.identifier.issn 1567-2379
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/156044
dc.description.abstract © 2019, The Author(s). Perineuronal net (PNN) is a highly structured portion of the CNS extracellular matrix (ECM) regulating synaptic plasticity and a range of pathologic conditions including posttraumatic regeneration and epilepsy. Here we studied Wisteria floribunda agglutinin-stained histological sections to quantify the PNN size and enrichment of chondroitin sulfates in mouse brain and spinal cord. Somatosensory cortex sections were examined during the period of PNN establishment at postnatal days 14, 21 and 28. The single cell PNN size and the chondroitin sulfate intensity were quantified for all cortex layers and specifically for the cortical layer IV which has the highest density of PNN-positive neurons. We demonstrate that the chondroitin sulfate proteoglycan staining intensity is increased between P14 and P28 while the PNN size remains unchanged. We then addressed posttraumatic changes of the PNN expression in laminae 6 and 7 of cervical spinal cord following hemisection injury. We demonstrate increase of the chondroitin sulfate content at 1.6–1.8 mm rostrally from the injury site and increase of the density of PNN-bearing cells at 0.4–1.2 mm caudally from the injury site. We further demonstrate decrease of the single cell PNN area at 0.2 mm caudally from the injury site suggesting that the PNN ECM takes part in the posttraumatic tissue rearrangement in the spinal cord. Our results demonstrate new insights on the PNN structure dynamics in the developing and posttraumatic CNS.
dc.relation.ispartofseries Journal of Molecular Histology
dc.subject Brain development
dc.subject Extracellular matrix
dc.subject Perineuronal nets
dc.subject Somatosensory cortex
dc.subject Spinal cord injury
dc.title Quantitative changes in perineuronal nets in development and posttraumatic condition
dc.type Article
dc.collection Публикации сотрудников КФУ
dc.source.id SCOPUS15672379-2019-SID85064071557


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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