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In vitro assessment of electrospun polyamide-6 scaffolds for esophageal tissue engineering

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dc.contributor.author Zhuravleva M.
dc.contributor.author Gilazieva Z.
dc.contributor.author Grigoriev T.
dc.contributor.author Shepelev A.
dc.contributor.author Kh. Tenchurin T.
dc.contributor.author Kamyshinsky R.
dc.contributor.author Krasheninnikov S.
dc.contributor.author Orlov S.
dc.contributor.author Caralogli G.
dc.contributor.author Archipova S.
dc.contributor.author Holterman M.
dc.contributor.author Mavlikeev M.
dc.contributor.author Deev R.
dc.contributor.author Chvalun S.
dc.contributor.author Macchiarini P.
dc.date.accessioned 2020-01-15T21:48:01Z
dc.date.available 2020-01-15T21:48:01Z
dc.date.issued 2019
dc.identifier.issn 1552-4973
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/156040
dc.description.abstract © 2018 Wiley Periodicals, Inc. Artificial tissue-engineered grafts offer a potential alternative to autologous tissue grafts for patients, which can be traumatic. After decellularizing Papio hamadryas esophagus and studying the morphology and physical properties of the extracellular matrix (ECM), we generated electrospun polyamide-6 based scaffolds to mimic it. The scaffolds supported a greater mechanical load than the native ECM and demonstrated similar 3D microstructure, with randomly aligned fibers, 90% porosity, 29 μm maximal pore size, and average fiber diameter of 2.87 ± 0.95 µm. Biocompatibility studies showed that human adipose- and bone marrow-derived mesenchymal stromal cells (AD-MSC and BMD-MSC) adhered to the scaffold surface and showed some proliferation: scaffold cell coverage was 25% after 72 h of incubation when seeded with 1000 cells/mm 2 ; cells elongated processes along the polyamide-6, although they flattened 1.67–4 times less than on cell culture plastic. Human umbilical vein endothelial cells, however, showed poor adherence and proliferation. We thus provide in vitro evidence that polyamide-6 scaffolds approximating the esophageal biomechanics and 3D topography of nonhuman primates may provide a biocompatible substrate for both AD-MSC and BMD-MSCs, supporting their adhesion and survival to some degree. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 253–268, 2019.
dc.relation.ispartofseries Journal of Biomedical Materials Research - Part B Applied Biomaterials
dc.subject biocompatibility
dc.subject electrospinning
dc.subject human mesenchymal stromal cells
dc.subject polyamide-6
dc.subject synthetic scaffolds
dc.title In vitro assessment of electrospun polyamide-6 scaffolds for esophageal tissue engineering
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 107
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 253
dc.source.id SCOPUS15524973-2019-107-2-SID85044643325


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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