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Blood clot contraction differentially modulates internal and external fibrinolysis

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dc.contributor.author Tutwiler V.
dc.contributor.author Peshkova A.
dc.contributor.author Le Minh G.
dc.contributor.author Zaitsev S.
dc.contributor.author Litvinov R.
dc.contributor.author Cines D.
dc.contributor.author Weisel J.
dc.date.accessioned 2020-01-15T21:47:53Z
dc.date.available 2020-01-15T21:47:53Z
dc.date.issued 2019
dc.identifier.issn 1538-7933
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/156022
dc.description.abstract © 2018 International Society on Thrombosis and Haemostasis Essentials Clot contraction influences the rate of fibrinolysis in vitro. Internal fibrinolysis is enhanced ∼2-fold in contracted vs. uncontracted blood clots. External fibrinolysis is ∼4-fold slower in contracted vs. uncontracted blood clots. Contraction can modulate lytic resistance and potentially the clinical outcome of thrombosis. Summary: Background Fibrinolysis involves dissolution of polymeric fibrin networks that is required to restore blood flow through vessels obstructed by thrombi. The efficiency of lysis depends in part on the susceptibility of fibrin to enzymatic digestion, which is governed by the structure and spatial organization of fibrin fibers. How platelet-driven clot contraction affects the efficacy of fibrinolysis has received relatively little study. Objective Here, we examined the effects of clot contraction on the rate of internal fibrinolysis emanating from within the clot to simulate (patho)physiological conditions and external fibrinolysis initiated from the clot exterior to simulate therapeutic thrombolysis. Methods Clot contraction was prevented by inhibiting platelet myosin IIa activity, actin polymerization or platelet-fibrin(ogen) binding. Internal fibrinolysis was measured by optical tracking of clot size. External fibrinolysis was determined by the release of radioactive fibrin degradation products. Results and Conclusions Clot contraction enhanced the rate of internal fibrinolysis ∼2-fold. In contrast, external fibrinolysis was ~4-fold slower in contracted clots. This dichotomy in the susceptibility of contracted and uncontracted clots to internal vs. external lysis suggests that the rate of lysis is dependent upon the interplay between accessibility of fibrin fibers to fibrinolytic agents, including clot permeability, and the spatial proximity of the fibrin fibers that modulate the effects of the fibrinolytic enzymes. Understanding how compaction of blood clots influences clot lysis might have important implications for prevention and treatment of thrombotic disorders.
dc.relation.ispartofseries Journal of Thrombosis and Haemostasis
dc.subject blood clotting
dc.subject clot retraction
dc.subject fibrin
dc.subject fibrinolysis
dc.subject platelets
dc.title Blood clot contraction differentially modulates internal and external fibrinolysis
dc.type Article
dc.relation.ispartofseries-issue 2
dc.relation.ispartofseries-volume 17
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 361
dc.source.id SCOPUS15387933-2019-17-2-SID85061271011


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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