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Renovation of glycomolecules for molecular imaging studies: Low-affinity glycan ligands can be used for selective cell imaging?

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dc.contributor.author Nomura S.
dc.contributor.author Tanaka K.
dc.date.accessioned 2020-01-15T21:18:43Z
dc.date.available 2020-01-15T21:18:43Z
dc.date.issued 2019
dc.identifier.issn 0385-5414
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/155643
dc.description.abstract © 2019 The Japan Institute of Heterocyclic Chemistry Since the interaction of single molecule of glycans is very weak to the lectins, in most cases they have been formed into clusters for binding studies. Then, a glycan molecule cannot be used for efficient cell imaging or targeting? We established a new strategy to selectively image the cells by using synergistic interaction of peptide and glycan ligands on cell surface. Namely, the peptide ligand with high affinity to the target surface receptor was pretargeted, and then the low-affinity glycan ligand with fluorescent label was interacted to the same cell; Bioorthogonal reaction between them can anchor the label on the target cells when both receptors are expressed and close in space on the surface. When the lectin is not expressed on the target or two receptors are separated, the weakly interacting glycan ligands are washed away the cells, hence the target cells are selectively imaged. While new idea and proof-of-concept data were exemplified based on our previous research, using this strategy, we would like to report the new data to visualize cell surface dynamics in response to the treatment with bioactive compounds.
dc.relation.ispartofseries Heterocycles
dc.title Renovation of glycomolecules for molecular imaging studies: Low-affinity glycan ligands can be used for selective cell imaging?
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 99
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 73
dc.source.id SCOPUS03855414-2019-99-1-SID85067510926


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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