Polymorphism of genes regulating the vitamin d hormonal system, and optimization of vitamin D intake in women with preeclampsia and a high risk for its development

Abstract

© 2019, Bionika Media Ltd. All rights reserved. Objective: to investigate the role of the polymorphism of genes involved in vitamin D metabolism in preeclampsia (PE) in women, by justifying the optimal dose of the vitamin to reduce the risk and severity of PE. Subjects and methods. Examinations were made in 226 patients: 30 healthy pregnant women, 35 patients with PE, 131 with high-risk PE, 63 at 14-16 weeks pregnant, and 68 who planned their pregnancy. GC rs2282679, CYP2R1 rs2060793, and VDR rs2228570 were genotyped. DNA was isolated from leukocytes by a PCR assay. Vitamin D intake was estimated from the blood level of 25(OH) D by enzyme immunoassay using the BIOMEDICAGRUPPE kits (Germany). Results. All the women were found to have vitamin D deficiency. In PE and at risk for its development, there was a genotype that did not ensure physiological metabolism of vitamin D. Conclusion. Prescribing vitamin D at a dose of 4000 IU to women at high risk for PE at the stage of pregravid preparation and during pregnancy provides a blood D vitamin level sufficient for the favorable course of pregnancy and childbirth.