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Synthesis and Characterization of Polyaspartic Acid-Histidine Conjugate as an Analog of Antioxidant Enzymes

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dc.contributor.author Kamalov M.
dc.contributor.author Sadrieva G.
dc.contributor.author Pavlyuk A.
dc.contributor.author Salakhieva D.
dc.contributor.author Petrova N.
dc.contributor.author Abdullin T.
dc.date.accessioned 2020-01-15T20:52:10Z
dc.date.available 2020-01-15T20:52:10Z
dc.date.issued 2019
dc.identifier.issn 0003-6838
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/155454
dc.description.abstract © 2019, Pleiades Publishing, Inc. Abstract: Polyamino acids are versatile macromolecules with potential to be used as bioactive compounds and drug carriers. Polyaspartic acid (pAsp) and amide conjugate of pAsp with L-histidine (pAsp–His) were synthesized and their catalytic antioxidant properties were studied. pAsp–His did not exhibit the ability to decompose hydrogen peroxide. According to the redox reaction of generation of superoxide radical (SOR) by NADH/phenazine methosulfate, pAsp–His at a concentration of ≥0.1 mg/mL showed superoxide dismutase (SOD)-like activity similar to that of SOD-2 (100 U/mL). In addition, we showed that L-histidine in composition with manganese (II) and copper (II) ions catalyzed SOR dismutation in proportion to the amino acid concentration. SOD-like activity of pAsp–His was observed without addition of metal cofactors. The conjugate caused some cytotoxic effect towards cancer cells (PC-3 line, IC50 ≈ 0.8 mg/mL, 72 h) and modulated viability human skin fibroblasts. The results are of particular interest for the development of bioactive peptides, which mimic action of natural enzymes.
dc.relation.ispartofseries Applied Biochemistry and Microbiology
dc.subject bioactive conjugates
dc.subject enzyme analogues
dc.subject histidine
dc.subject polyaspartic acid
dc.subject superoxide dismutase
dc.subject synthetic peptides
dc.title Synthesis and Characterization of Polyaspartic Acid-Histidine Conjugate as an Analog of Antioxidant Enzymes
dc.type Article
dc.relation.ispartofseries-issue 5
dc.relation.ispartofseries-volume 55
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 474
dc.source.id SCOPUS00036838-2019-55-5-SID85073220986


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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