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Electrochemical Acetylcholinesterase Biosensor Based on Polylactide–Nanosilver Composite for the Determination of Anti-dementia Drugs

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dc.contributor.author Shamagsumova R.
dc.contributor.author Yu. Efimova O.
dc.contributor.author Gorbatchuk V.
dc.contributor.author Evtugyn V.
dc.contributor.author Stoikov I.
dc.contributor.author Evtugyn G.
dc.date.accessioned 2020-01-15T20:52:08Z
dc.date.available 2020-01-15T20:52:08Z
dc.date.issued 2019
dc.identifier.issn 0003-2719
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/155452
dc.description.abstract © 2019, © 2019 Taylor & Francis Group, LLC. A new acetylcholinesterase biosensor has been developed for the determination of anticholinesterase drugs applied for neurodegenerative disease treatment. For this purpose, silver nanosendrites were deposited by potentiostatic electrolysis on a glassy carbon electrode covered with oligolactides cross-linked with p-tert-butylthiacliax[4]arene core in the cone, partial cone, and 1,3-alternate configurations. The roles of macrocycle configuration and electrolysis conditions on the silver depostion were characterized and optimal conditions selected for the subsequent immobilization of acetylcholinesterase. Silver nanoparticles provide higher response at low working potential (0.05 V) due to the electrostatic accumulation of silver ions and prevention of their leaching after reoxidation. The biosensor allows the determination of 10−12–10−7 M donepezil, berberine, and huperzine A within 20-30 s by the relative decay of the current related to the oxidation of thiocholine formed in enzymatic reaction. The reversible inhibition of immobilized acetylcholinesterase with huperzine A was quantified for the first time. The developed biosensor was employed for the analysis of spiked urine samples.
dc.relation.ispartofseries Analytical Letters
dc.subject Acetylcholinesterase biosensor
dc.subject enzyme inhibitor
dc.subject oligolactic acid
dc.subject p-tert-butylthiacalix[4]arene
dc.subject silver nanoparticles
dc.title Electrochemical Acetylcholinesterase Biosensor Based on Polylactide–Nanosilver Composite for the Determination of Anti-dementia Drugs
dc.type Article
dc.relation.ispartofseries-issue 10
dc.relation.ispartofseries-volume 52
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 1558
dc.source.id SCOPUS00032719-2019-52-10-SID85060568952


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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