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Combined use of plasmid drug pCMV-VEGFA and autodermoplasty for stimulation of skin defects healing in the experiment

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dc.contributor.author Bilialov A.
dc.contributor.author Abyzova M.
dc.contributor.author Titova A.
dc.contributor.author Mavlikeev M.
dc.contributor.author Krilov A.
dc.contributor.author Bozo I.
dc.contributor.author Deev R.
dc.date.accessioned 2019-01-22T20:58:12Z
dc.date.available 2019-01-22T20:58:12Z
dc.date.issued 2018
dc.identifier.issn 2313-1829
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/149666
dc.description.abstract © 2018 Human Stem Cell Institute. All rights reserved. To find effective ways to stimulate chronic skin wounds healing (including deep burns, diabetic and trophic ulcers) is an actual multidisciplinary task. The aim of our study was to assess the potential of using autodermoplasty in combination with plasmid drug pCMV-VEGFA to optimize skin defects repair in the experiment. Autodermoplasty was performed on Wistar rats. The size of the skin flap was 22 cm. Immediately after surgery the animals of the test group (n=8) underwent intradermal injection in the periphery of autotransplant with 1 ml solution containing 0.3 mg of supercoiled plasmid DNA pCMV-VEGFA, rats of the control group (n=8) received 1 ml of 0.9 % NaCl. The results were analyzed in 3, 6, 9 12, 18 days using macroscopic evaluation, laser Doppler flowmetry, histological methods. Macroscopically in the test group necrosis of the transplanted skin flap was found at later periods of observation, in one case complete survival of autotransplant was observed. The results of laser Doppler flowmetry in the group with plasmid DNA did not have statistically significant differences with control. The wound defect diameter in the test group at 12 days was 5,52± 4.80 mm, in the control 12,45±0,82 mm (p=0,03); 2,53±of 2,94 mm and 4,23±3,5 mm (p=0,067) at 18 days, respectively. At 18 days, the average number of vessels under the flap in the central zone were: of 26±2,9 in the test group and 20±8 in control; it the peripheral zone 27±3,4 and of 12,1±3,9 (p=0,035), respectively; in the skin muscle 21,2±of 3,9 and 12,4±3,6 (p=0,04), respectively. Thus, the use of plasmid drug pCMV-VEGFA improved the skin healing after autodermoplasty.
dc.relation.ispartofseries Genes and Cells
dc.subject Autodermoplasty
dc.subject Gene Therapy
dc.subject Skin
dc.subject Vascular Endothelial Growth factor
dc.subject VEGFA gene
dc.title Combined use of plasmid drug pCMV-VEGFA and autodermoplasty for stimulation of skin defects healing in the experiment
dc.type Article
dc.relation.ispartofseries-issue 1
dc.relation.ispartofseries-volume 13
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 90
dc.source.id SCOPUS23131829-2018-13-1-SID85049149907


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  • Публикации сотрудников КФУ Scopus [22633]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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