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Calcium Transient and Quantal Release in Mouse Neuromuscular Junction Under Extracellular Calcium Concentration Change

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dc.contributor.author Samigullin D.
dc.contributor.author Zhilyakov N.
dc.contributor.author Khaziev E.
dc.contributor.author Bukharaeva E.
dc.contributor.author Nikolsky E.
dc.date.accessioned 2019-01-22T20:57:33Z
dc.date.available 2019-01-22T20:57:33Z
dc.date.issued 2018
dc.identifier.issn 2191-1630
dc.identifier.uri https://dspace.kpfu.ru/xmlui/handle/net/149616
dc.description.abstract © 2018, Springer Science+Business Media, LLC, part of Springer Nature. In mouse neuromuscular junction, the amplitude of the presynaptic calcium (Ca2+) transient was measured and correlated with mediator release at different extracellular Ca2+ concentrations. Fluorescent calcium-sensitive dye Oregon Green 488 BAPTA 1 hexapotassium salt was used for Ca2+ transient registration. The quantal content of release was assessed by the amplitude of the endplate potentials (EPPs) and was measured using intracellular microelectrodes. The amplitude of the EPPs changed more significantly than the amplitude of the Ca2+ transient when the extracellular calcium concentration was changed. Linear approximation of the dependence of the quantal content on the amplitude of the Ca2+ transient on double logarithmic scale gave a slope showing that the biochemical cooperativity was 2.86. The obtained value is comparable with the data calculated earlier in the neuromuscular junction of the rat and other synapses using electrophysiological measurements. Our data suggest that the change of the Ca2+ transients recorded from the whole volume of the nerve terminal properly reflects the variation of calcium concentration responsible for the neurotransmitter release in active zone. Thus, analysis of the bulk Ca2+ transient can be used to evaluate the calcium entry into the nerve endings and compare it with the number of quanta released under different conditions.
dc.relation.ispartofseries BioNanoScience
dc.subject Calcium transient
dc.subject Fluorescence dyes
dc.subject Mediator release
dc.subject Neuromuscular junction
dc.subject Optical imaging
dc.subject Quantal content
dc.title Calcium Transient and Quantal Release in Mouse Neuromuscular Junction Under Extracellular Calcium Concentration Change
dc.type Article
dc.relation.ispartofseries-issue 4
dc.relation.ispartofseries-volume 8
dc.collection Публикации сотрудников КФУ
dc.relation.startpage 984
dc.source.id SCOPUS21911630-2018-8-4-SID85056329350


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  • Публикации сотрудников КФУ Scopus [24551]
    Коллекция содержит публикации сотрудников Казанского федерального (до 2010 года Казанского государственного) университета, проиндексированные в БД Scopus, начиная с 1970г.

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